FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n02_DAM-GR-EV-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n02_DAM-GR-EV-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13575398
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC11697528.616705012994831Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG3137542.3111955907296418Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC1746061.2861943347811977Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT1334310.9828883101622509Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC1217990.8972038978157398Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG1160060.854531115772812Clontech Universal Primer Mix Long (100% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC1106700.8152247175368265Clontech Universal Primer Mix Long (100% over 23bp)
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA1002180.7382324997027712Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA993430.7317870164837893Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC808630.5956584108988922Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC789060.5812426272879808Clontech Universal Primer Mix Long (95% over 24bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG731380.5387540019084524Clontech Universal Primer Mix Long (95% over 23bp)
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG684000.5038526310609825Clontech Universal Primer Mix Long (95% over 23bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA670910.49421018816538564No Hit
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG635590.46819253476030687Clontech Universal Primer Mix Long (95% over 24bp)
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG601760.44327245506908897Clontech Universal Primer Mix Long (95% over 23bp)
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC557830.410912446176532Clontech Universal Primer Mix Long (95% over 23bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT551430.4061980355935053Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTCTAATA538390.3965924240305883Clontech Universal Primer Mix Long (95% over 23bp)
CGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTC517890.3814915776318308Clontech Universal Primer Mix Long (95% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA502170.3699118066372713No Hit
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG502020.3698013126392316Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA483220.35595273155159063Clontech Universal Primer Mix Long (95% over 21bp)
GGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAG480040.35361025879314917Clontech Universal Primer Mix Long (95% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC441310.32508070849930143Clontech Universal Primer Mix Long (100% over 23bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA433660.31944551459927734No Hit
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC410020.3020316605082223Clontech Universal Primer Mix Long (100% over 23bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC396060.29174835242399527No Hit
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT391850.288647154212348Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA347150.2557199427965206Clontech Universal Primer Mix Long (100% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTCTAATA328300.24183453037619965Clontech Universal Primer Mix Long (95% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG302890.2231168471082763No Hit
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA294100.21664189882315052Clontech Universal Primer Mix Long (95% over 23bp)
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT284560.2096144805478263No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG273570.20151895362478506Clontech Universal Primer Mix Long (95% over 23bp)
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG271400.1999204737864776Clontech Universal Primer Mix Long (100% over 23bp)
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC269890.19880816753954472Clontech Universal Primer Mix Long (100% over 23bp)
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA260520.1919059757953321Clontech Universal Primer Mix Short (100% over 21bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC257370.18958560183649864Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTCTAAT255510.1882154762608065Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC248650.1831622174171247Clontech Universal Primer Mix Long (95% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC243180.17913286962194405No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT235040.1731367286616569No Hit
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC229540.16908528206686832Clontech Universal Primer Mix Short (100% over 22bp)
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA223630.16473181854410457No Hit
GGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCG215470.15872094505074547Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATCTAATA202540.14919636241972428Clontech Universal Primer Mix Long (95% over 23bp)
TCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGC195760.14420203370833032Clontech Universal Primer Mix Long (95% over 23bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG193300.14238993214047943Clontech Universal Primer Mix Long (95% over 22bp)
GTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAT189830.1398338376524946No Hit
GTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG184200.13568662959273828Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA176400.12994094169467443Clontech Universal Primer Mix Long (95% over 23bp)
GCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCG175140.12901279211114106Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAGATCGG173300.12765739906852086Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG172520.12708283027871448Clontech Universal Primer Mix Long (100% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCT171650.1264419650900843Clontech Universal Primer Mix Long (95% over 24bp)
ATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAT171280.12616941322825304Clontech Universal Primer Mix Long (100% over 23bp)
AGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGG164610.12125611344875487Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAA158150.11649750526651226Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCC157600.1160923606070334Clontech Universal Primer Mix Long (100% over 23bp)
TAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCA157020.11566511714794661Clontech Universal Primer Mix Long (100% over 23bp)
AGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAG156780.1154883267510831Clontech Universal Primer Mix Long (95% over 23bp)
GGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCG152860.11260075026897921Clontech Universal Primer Mix Long (95% over 24bp)
CTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGT151170.11135585122439871No Hit
TAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAG148270.10921963392896473No Hit
AGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTA144200.10622156344882118No Hit
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG137390.10120513593781927Clontech Universal Primer Mix Long (95% over 24bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)