FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6145264
Sequences flagged as poor quality0
Sequence length76
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC2301673.745437136630745Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG727851.184408025432268Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC504690.8212665883841606Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC422070.6868215913913545Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT346130.563246753923021Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC319240.5194894800288482Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG308920.5026960599251716Clontech Universal Primer Mix Long (100% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC255270.4153930571575119Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG210660.34280056967446804Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC196260.31936789046003555Clontech Universal Primer Mix Long (95% over 24bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA194020.3157228070266794No Hit
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT132990.216410556161623No Hit
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT128980.2098852059081595Clontech Universal Primer Mix Long (95% over 24bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG120410.19593950723679243Clontech Universal Primer Mix Long (95% over 23bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG110720.18017126684874726Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA106430.17319028116611426Clontech Universal Primer Mix Long (95% over 23bp)
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG106050.17257191879795564Clontech Universal Primer Mix Long (100% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC105570.17179082949080787Clontech Universal Primer Mix Long (100% over 23bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC103660.1686827449561158No Hit
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA102750.16720192981131485Clontech Universal Primer Mix Long (95% over 21bp)
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG100760.1639636637254315Clontech Universal Primer Mix Long (95% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC95410.1552577724895139Clontech Universal Primer Mix Long (100% over 23bp)
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA93890.15278432301687933Clontech Universal Primer Mix Long (95% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA92000.1497087838699851No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA81930.13332218111378127Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACT81550.13270381874562265Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA71670.11662639717349817Clontech Universal Primer Mix Long (100% over 22bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC69050.11236295137198338Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA68220.11101231777837371Clontech Universal Primer Mix Long (95% over 23bp)
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA65270.10621187307819485Clontech Universal Primer Mix Short (100% over 21bp)
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC64540.10502396642357431Clontech Universal Primer Mix Long (100% over 23bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA64310.10464969446389936No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAA62640.10193215458278114Clontech Universal Primer Mix Long (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)