FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n01_dam126-GR-bZIP1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n01_dam126-GR-bZIP1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12812506
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC6455105.038124469951468Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC2605572.0336146574292338Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG2243151.7507503996485934Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC2024171.5798392601728342Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT1595671.2454003923978652Clontech Universal Primer Mix Long (95% over 24bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA1388131.0834180292286302No Hit
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC1343951.048936094156756Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG1319831.0301107371188742Clontech Universal Primer Mix Long (100% over 23bp)
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT1235990.9646746702011301No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC1111160.8672464231431384Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC1109680.8660913017328539Clontech Universal Primer Mix Long (95% over 23bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC723270.5645031502814516No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG713840.5571431537280841Clontech Universal Primer Mix Long (95% over 23bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT597610.46642709864877335Clontech Universal Primer Mix Long (95% over 24bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG569370.44438613336064Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG549750.4290729697999751Clontech Universal Primer Mix Long (95% over 24bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG522460.4077734675792542Clontech Universal Primer Mix Long (95% over 24bp)
TAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAG512070.39966420308408046No Hit
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA500580.3906964024055872Clontech Universal Primer Mix Long (95% over 21bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA477390.3725968986863304No Hit
CTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCT456600.35637056482158913Clontech Universal Primer Mix Long (95% over 24bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC441750.344780326346774Clontech Universal Primer Mix Long (100% over 23bp)
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG436360.34057349904850776Clontech Universal Primer Mix Long (100% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCC425410.3320271615872804Clontech Universal Primer Mix Long (100% over 23bp)
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG418360.3265247251396409Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGG416330.3249403356376965Clontech Universal Primer Mix Long (100% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC411830.32142814216047977Clontech Universal Primer Mix Long (100% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCT399880.31210131725987095Clontech Universal Primer Mix Long (95% over 24bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCC396800.30969741594657596Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGG387280.30226717552366417Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA384370.299995957075064Clontech Universal Primer Mix Long (95% over 23bp)
ATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCTATAGTGA357390.27893840596055136No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGC356340.2781188941492008Clontech Universal Primer Mix Long (95% over 24bp)
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA335060.26151012143916263Clontech Universal Primer Mix Short (100% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGGC334960.2614320726952245Clontech Universal Primer Mix Long (95% over 24bp)
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA329600.2572486600201397Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA319040.24900671266027113Clontech Universal Primer Mix Long (100% over 22bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC304420.23759598629651374Clontech Universal Primer Mix Long (95% over 24bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA294310.22970525828436686No Hit
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG288250.22497550440171502Clontech Universal Primer Mix Long (100% over 23bp)
ATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCTATAGTGA281580.21976965318104047No Hit
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC279250.21795111744728157Clontech Universal Primer Mix Long (100% over 23bp)
CAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTG271540.21193355928965027Clontech Universal Primer Mix Long (95% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG262170.2046203919826457No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG241430.18843308248987356Clontech Universal Primer Mix Long (100% over 23bp)
AGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT228770.17855211150730388No Hit
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTA228480.17832577014988324No Hit
CTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCT227820.17781064843989147Clontech Universal Primer Mix Long (95% over 24bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCC225800.17623406381234086Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA212460.16582236137099174Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTCTAATA209950.1638633378981442Clontech Universal Primer Mix Long (95% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC207980.16232577764256267No Hit
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATACGA206880.16146724145924302Clontech Universal Primer Mix Long (95% over 21bp)
CTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGT198260.15473943973177456No Hit
CGTATTAGCTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA198240.15472382998298692Clontech Universal Primer Mix Long (100% over 22bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGC197780.15436480576087147Clontech Universal Primer Mix Long (95% over 24bp)
CAGCGTGGTCGCGGCCGAGGAGCTAATACGACTCACTATAGGGCAGCGTG194500.15180480695970014Clontech Universal Primer Mix Long (95% over 23bp)
CGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTC193500.15102431952031867Clontech Universal Primer Mix Long (95% over 23bp)
ATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCTATAGTGA193360.15091505127880525No Hit
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT188990.14750432116870812Clontech Universal Primer Mix Long (95% over 23bp)
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC188910.1474418821735576Clontech Universal Primer Mix Short (100% over 22bp)
CGTATTAGTTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA187970.146708223980539Clontech Universal Primer Mix Long (95% over 23bp)
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC182690.14258725030060473Clontech Universal Primer Mix Long (95% over 23bp)
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG182220.14222042120409542Clontech Universal Primer Mix Long (95% over 23bp)
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA180940.14122139728168712No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT171110.13354920575256707No Hit
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA168680.13165262127487004Clontech Universal Primer Mix Long (95% over 23bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCG166430.1298965245362617Clontech Universal Primer Mix Long (95% over 24bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATACGACTCACTA166330.12981847579232353No Hit
CCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCT165140.12888969573945955Clontech Universal Primer Mix Long (95% over 24bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCG161150.1257755508563274Clontech Universal Primer Mix Long (95% over 24bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATACGA159510.12449555145574176Clontech Universal Primer Mix Long (95% over 21bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCT158670.1238399420066613Clontech Universal Primer Mix Long (95% over 24bp)
ATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAT158370.12360579577484684Clontech Universal Primer Mix Long (100% over 23bp)
AGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT155970.12173262592033128No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTAATACG149400.11660482344359488Clontech Universal Primer Mix Long (95% over 23bp)
GTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC148930.11623799434708558No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA145920.11388872715454729Clontech Universal Primer Mix Long (95% over 24bp)
GTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC145080.11323311770546683No Hit
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC142910.111539459962009Clontech Universal Primer Mix Long (95% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGG141440.11039214342611821No Hit
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAG140470.10963507060991816Clontech Universal Primer Mix Long (100% over 23bp)
CTCGGCCGGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCG140250.10946336337325421Clontech Universal Primer Mix Long (95% over 24bp)
TAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCA140080.10933068050855937Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGC137510.10732482778934894Clontech Universal Primer Mix Long (100% over 23bp)
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG137210.10709068155753448Clontech Universal Primer Mix Long (95% over 22bp)
GGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCG135530.10577946265937357Clontech Universal Primer Mix Long (95% over 23bp)
GTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAT135150.10548287743240863No Hit
CCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAG132850.10368775632183119No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG132330.10328190285335281Clontech Universal Primer Mix Long (95% over 22bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG131950.10298531762638784Clontech Universal Primer Mix Long (95% over 24bp)
TCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGC131270.10245458616760841Clontech Universal Primer Mix Long (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)