FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n01_dam126-GR-EV-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n01_dam126-GR-EV-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13945891
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC4509253.2333896772891744Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG3545902.5426127308753528Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG1754771.2582702675648332Clontech Universal Primer Mix Long (100% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT1640361.176231766044923Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC1071400.768254964849503Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC1019480.731025360803408Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC747610.536079050094397Clontech Universal Primer Mix Long (95% over 23bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT741530.5317193429950083Clontech Universal Primer Mix Long (95% over 24bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC726740.5211140686529101Clontech Universal Primer Mix Long (95% over 24bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC717890.5147681134177802Clontech Universal Primer Mix Long (100% over 23bp)
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG667780.47883638270225976Clontech Universal Primer Mix Long (95% over 23bp)
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG658040.4718522466581733Clontech Universal Primer Mix Long (100% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC643540.46145491887180246Clontech Universal Primer Mix Long (100% over 23bp)
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA628420.4506130156904281Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA577900.41438729156853443Clontech Universal Primer Mix Long (95% over 21bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG575660.41278108368981226Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC567350.40682233928258865Clontech Universal Primer Mix Long (95% over 23bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG513380.36812276820462747Clontech Universal Primer Mix Long (95% over 24bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC499180.35794055754487114Clontech Universal Primer Mix Long (95% over 24bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA484210.34720621292680404No Hit
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC451150.3235003055738784Clontech Universal Primer Mix Long (100% over 23bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA451090.3234572821485554Clontech Universal Primer Mix Long (100% over 22bp)
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG441400.31650899895890483Clontech Universal Primer Mix Long (95% over 23bp)
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA435840.3125221615456481Clontech Universal Primer Mix Short (100% over 21bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA430260.3085209829906171No Hit
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG410510.2943591054884912Clontech Universal Primer Mix Long (100% over 23bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC405160.2905228500638647No Hit
GTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAT368740.2644076308928558No Hit
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA353190.2532573931633339No Hit
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA344740.2471982607636902Clontech Universal Primer Mix Long (95% over 23bp)
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC343690.2464453508205392Clontech Universal Primer Mix Long (95% over 23bp)
GCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGA330170.23675073898110918Clontech Universal Primer Mix Long (100% over 23bp)
GGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCG314970.22585147123263763Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG290250.208125819999597Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA288900.20715779292983144Clontech Universal Primer Mix Long (95% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG285100.20443297599271357No Hit
CCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAG278700.19984381062493603No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG272800.19561317380151613Clontech Universal Primer Mix Long (95% over 22bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT271960.19501084584699538No Hit
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT270150.19371297251642078Clontech Universal Primer Mix Long (95% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC266440.19105269071728728No Hit
GAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGT259830.18631294336087956No Hit
AGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTA259610.18615519080136222No Hit
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC253740.1819460656906038Clontech Universal Primer Mix Short (100% over 22bp)
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA238700.17116152707632665No Hit
ATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAT238410.17095358052059922Clontech Universal Primer Mix Long (100% over 23bp)
GGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCG238120.1707456339648718Clontech Universal Primer Mix Long (95% over 23bp)
TCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGC232940.16703127824532688Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCT232080.16641460914903178Clontech Universal Primer Mix Long (95% over 24bp)
AGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGG230320.16515258867289298Clontech Universal Primer Mix Long (95% over 23bp)
CTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGT224100.1606924935810842No Hit
CGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTC221090.15853415174405136Clontech Universal Primer Mix Long (95% over 23bp)
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT220730.15827601119211387No Hit
GACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACG219590.1574585661109785Clontech Universal Primer Mix Long (95% over 24bp)
GTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG216650.1553504182701557Clontech Universal Primer Mix Long (95% over 23bp)
TAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCA214750.15398800980159674Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGG214680.1539378158053867Clontech Universal Primer Mix Long (100% over 23bp)
CGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCC210610.15101939345431567Clontech Universal Primer Mix Long (95% over 24bp)
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC209430.1501732660896317Clontech Universal Primer Mix Long (95% over 23bp)
GCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTAT207520.1488036870501856Clontech Universal Primer Mix Short (100% over 22bp)
GGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAG201540.1445156856596685Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG200060.14345444116836994Clontech Universal Primer Mix Long (95% over 24bp)
CTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCT198630.14242904953150717Clontech Universal Primer Mix Long (95% over 24bp)
TATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGA194150.1392166337740629Clontech Universal Primer Mix Long (100% over 23bp)
AGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAG194060.13915209863607855Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGG194030.13913058692341707Clontech Universal Primer Mix Long (100% over 23bp)
TGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTA189420.13582495374443984Clontech Universal Primer Mix Long (100% over 23bp)
ACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAG188810.13538754892032354No Hit
AGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCT186840.13397494645555455Clontech Universal Primer Mix Long (100% over 23bp)
GATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTC182920.1311640826677908Clontech Universal Primer Mix Long (100% over 23bp)
TGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGC178160.12775089092550632Clontech Universal Primer Mix Long (95% over 23bp)
TATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGG169540.12156985882078097Clontech Universal Primer Mix Short (100% over 21bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG166550.11942585812552242Clontech Universal Primer Mix Long (95% over 23bp)
ACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGC157270.11277156834224504Clontech Universal Primer Mix Long (100% over 23bp)
TATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTG155640.1116027652876392No Hit
GACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCAC152990.10970256400254384No Hit
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCC152580.10940857059617058Clontech Universal Primer Mix Long (100% over 23bp)
GGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGC152090.10905721262270013Clontech Universal Primer Mix Long (95% over 23bp)
TAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCC151260.10846205523906648Clontech Universal Primer Mix Long (100% over 23bp)
TCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTAT150120.10764461015793111No Hit
TAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAG148880.10675545936792422No Hit
TTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATC148500.10648297767421241Clontech Universal Primer Mix Long (100% over 23bp)
TCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCG147640.10586630857791733Clontech Universal Primer Mix Long (95% over 24bp)
CAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTG147530.10578743229815865Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCT141480.10144923691143147Clontech Universal Primer Mix Long (95% over 24bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)