FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n01_dam126-GR-EV-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n01_dam126-GR-EV-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17453960
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC7568804.336437117994999Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG3217031.8431519265542033Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT1767001.0123777068355833Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG1614320.9249018560830895Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC1598120.9156202947640536Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC1502140.8606299086281852Clontech Universal Primer Mix Long (100% over 23bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA1479540.8476815576522462No Hit
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT1463420.8384458312039217No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC1445260.8280413155524592Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC1297800.7435561901138767Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC1094510.6270840542776539Clontech Universal Primer Mix Long (95% over 23bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC816690.46791100701502697No Hit
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT705140.40400000916697415Clontech Universal Primer Mix Long (95% over 24bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG691460.39616224627534385Clontech Universal Primer Mix Long (95% over 23bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG664480.3807044361279618Clontech Universal Primer Mix Long (95% over 24bp)
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG644970.3695264570332463Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA623370.3571510419411984Clontech Universal Primer Mix Long (95% over 21bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA606470.34746842550343876No Hit
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG606390.34742259063272746Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG554520.31770440633529584Clontech Universal Primer Mix Long (95% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC531050.3042576011403716Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGCTAATA523930.30017829764706694Clontech Universal Primer Mix Long (95% over 23bp)
TAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAG507470.2907477729982193No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA507340.2906732913333135Clontech Universal Primer Mix Long (95% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC480180.27511235272683104Clontech Universal Primer Mix Long (100% over 23bp)
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG453610.25988944629184435Clontech Universal Primer Mix Long (95% over 24bp)
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA444820.2548533398724415Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA419970.2406158831577476Clontech Universal Primer Mix Long (100% over 22bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA411350.23567717583860623No Hit
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA389550.22318717356978016Clontech Universal Primer Mix Short (100% over 21bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC372100.21318944239588034Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGGCTAATA336540.19281584236471264Clontech Universal Primer Mix Long (95% over 23bp)
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG333750.19121735124865646Clontech Universal Primer Mix Long (100% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG330760.18950427295582206No Hit
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC322820.1849551620377267Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA318670.18257747811957858Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG314720.1803143813782087Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTCTAATA306180.17542150892977867Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTCTAATA295680.16940568214892207Clontech Universal Primer Mix Long (95% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC279530.16015276762408073No Hit
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC272770.15627972104897686Clontech Universal Primer Mix Long (95% over 23bp)
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG271600.15560938606482425Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCT271030.15528281261100632Clontech Universal Primer Mix Long (95% over 24bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCC269980.15468122993292066Clontech Universal Primer Mix Long (100% over 23bp)
CGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTC269560.1544405968616864Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCT263030.1506993255398775Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGG254560.14584655860331983Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATGCTAATA253360.14515903554265047Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGC245180.14047242001242125Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGG244740.14022032822350916Clontech Universal Primer Mix Long (100% over 23bp)
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA243970.139779167592913Clontech Universal Primer Mix Long (95% over 23bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT243960.1397734382340741No Hit
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA243870.1397218740045239No Hit
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT242920.13917758491482735Clontech Universal Primer Mix Long (95% over 23bp)
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC239240.13706918086210806Clontech Universal Primer Mix Short (100% over 22bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCC228100.13068667511556117Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA225980.12947205104171203Clontech Universal Primer Mix Long (95% over 24bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCC225340.1291053720760217Clontech Universal Primer Mix Long (100% over 23bp)
ATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCTATAGTGA221390.12684227533465187No Hit
CTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGT217890.12483699974103298No Hit
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG212730.12188065058015486Clontech Universal Primer Mix Long (95% over 24bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGC204240.11701642492591938Clontech Universal Primer Mix Long (95% over 24bp)
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC201900.1156757549576142Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCT201440.11541220445102429Clontech Universal Primer Mix Long (95% over 24bp)
GTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAT201380.11537782829799083No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGGC198880.11394548858826307Clontech Universal Primer Mix Long (95% over 24bp)
ATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCTATAGTGA198330.11363037385212296No Hit
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAG186260.10671503773355731Clontech Universal Primer Mix Long (100% over 23bp)
TCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGC179860.10304824807665423Clontech Universal Primer Mix Long (95% over 23bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG175430.10051014211101665Clontech Universal Primer Mix Long (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)