FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n01_GR-bZIP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n01_GR-bZIP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12221771
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC3310912.709026375964662Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG1833131.4998890095387976Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG951500.7785287418656429Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC874220.7152973165672961Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT812660.6649281843032405Clontech Universal Primer Mix Long (95% over 24bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC560830.45887785002680875Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC526550.43082954180699345Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC426710.349139253222794Clontech Universal Primer Mix Long (95% over 24bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA414940.33950889768757736Clontech Universal Primer Mix Long (95% over 21bp)
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG392440.3210991271232295Clontech Universal Primer Mix Long (100% over 23bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT363860.2977146274463824Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC361020.2953909052951491Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA346740.28370683757697635Clontech Universal Primer Mix Long (95% over 23bp)
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG337760.2763592935917389Clontech Universal Primer Mix Long (95% over 23bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG333570.27293098520664477Clontech Universal Primer Mix Long (95% over 23bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA332530.27208004470055935No Hit
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA329840.26987905435308845No Hit
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA329660.2697317761885737Clontech Universal Primer Mix Long (95% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC306550.25082289628892573Clontech Universal Primer Mix Long (100% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC298900.24456357429704745Clontech Universal Primer Mix Long (100% over 23bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG295670.2419207494560322Clontech Universal Primer Mix Long (95% over 24bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC274100.22427191607501074No Hit
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA263640.2157134182926517Clontech Universal Primer Mix Short (100% over 21bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA257000.2102804904461064No Hit
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA254950.208603155794688Clontech Universal Primer Mix Long (100% over 22bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC234320.19172344171724376Clontech Universal Primer Mix Long (95% over 24bp)
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG225750.18471136466228993Clontech Universal Primer Mix Long (95% over 23bp)
GTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAT210940.17259364457082366No Hit
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC207900.170106280014574Clontech Universal Primer Mix Long (100% over 23bp)
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA207120.16946807463500993Clontech Universal Primer Mix Long (95% over 23bp)
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG199520.16324966324438578Clontech Universal Primer Mix Long (100% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG198990.16281601087109224No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT198930.16276691814958733No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC187810.1536684004306741No Hit
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC183410.15006826752031271Clontech Universal Primer Mix Long (95% over 23bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG178700.14621448888217592Clontech Universal Primer Mix Long (95% over 22bp)
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC170590.1395787893587599Clontech Universal Primer Mix Long (95% over 23bp)
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA169490.13867875613116953No Hit
CCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAG167180.13678868635322983No Hit
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT157710.12904021847570207No Hit
AGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTA157240.12865565882391347No Hit
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC156820.128312009773379Clontech Universal Primer Mix Short (100% over 22bp)
GCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGA156210.12781290043807889Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG152920.1251209828755587Clontech Universal Primer Mix Long (100% over 23bp)
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT150990.1235418336671502Clontech Universal Primer Mix Long (95% over 23bp)
GGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCG150300.12297726736984355Clontech Universal Primer Mix Long (95% over 24bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCTAATACGACTCA141590.11585064063137822Clontech Universal Primer Mix Long (95% over 24bp)
CTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGT139160.11386238541042865No Hit
GAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGT139020.11374783572691716No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG131250.10739032829202905Clontech Universal Primer Mix Long (95% over 23bp)
GGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCG130090.10644120234293378Clontech Universal Primer Mix Long (95% over 23bp)
ACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAG124390.10177739379996564No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)