FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n01_DAM-GR-bZIP1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n01_DAM-GR-bZIP1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13562475
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC4939623.642122842622751Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG3772952.781903745444692Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG1941991.4318846670685108Clontech Universal Primer Mix Long (100% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT1910001.4082975268157176Clontech Universal Primer Mix Long (95% over 24bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC1208380.8909730709181031Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC995140.733745131327431Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC908600.6699367187773618Clontech Universal Primer Mix Long (95% over 24bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT814780.6007605543973352Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC787900.5809411630251853Clontech Universal Primer Mix Long (95% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC765120.5641448187001267Clontech Universal Primer Mix Long (100% over 23bp)
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG723360.5333539785326793Clontech Universal Primer Mix Long (100% over 23bp)
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG698260.5148470319760958Clontech Universal Primer Mix Long (95% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC686490.5061686749652994Clontech Universal Primer Mix Long (100% over 23bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG674380.497239626248159Clontech Universal Primer Mix Long (95% over 23bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG667580.4922257921212758Clontech Universal Primer Mix Long (95% over 24bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA653930.4821612574401059No Hit
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA651310.4802294566441597Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA582570.4295454922497553Clontech Universal Primer Mix Long (95% over 21bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC541560.3993076484933613Clontech Universal Primer Mix Long (95% over 23bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC535850.3950975024838756Clontech Universal Primer Mix Long (95% over 24bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA479260.3533720799485345Clontech Universal Primer Mix Long (100% over 22bp)
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC473040.3487858963795325Clontech Universal Primer Mix Long (100% over 23bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC464350.34237851129679503No Hit
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA459060.33847804327749914No Hit
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA443470.32698309121307134Clontech Universal Primer Mix Short (100% over 21bp)
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG427340.3150899817326852Clontech Universal Primer Mix Long (95% over 23bp)
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG417150.30757660382784113Clontech Universal Primer Mix Long (100% over 23bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA355840.2623709905456047No Hit
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC355140.26185486056195495Clontech Universal Primer Mix Long (95% over 23bp)
GTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAT354030.26103642587359605No Hit
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA336470.24808893656946834Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG320150.2360557346649487Clontech Universal Primer Mix Long (100% over 23bp)
GCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGA308240.2272741516574224Clontech Universal Primer Mix Long (100% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG294390.21706215126663825No Hit
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT291150.21467320677088808Clontech Universal Primer Mix Long (95% over 23bp)
GGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCG289000.21308795039253528Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA278220.2051395486443293Clontech Universal Primer Mix Long (95% over 23bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT265730.19593031507892178No Hit
ATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAT264100.19472846954556597Clontech Universal Primer Mix Long (100% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCT259990.19169804921299394Clontech Universal Primer Mix Long (95% over 24bp)
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC256130.18885196101743967Clontech Universal Primer Mix Short (100% over 22bp)
CGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTC252780.18638191038140162Clontech Universal Primer Mix Long (95% over 23bp)
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT252300.1860279926783275No Hit
GAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGT248400.1831524113408504No Hit
CCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAG246310.1816113946753819No Hit
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC245550.1810510249788479No Hit
TCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGC240630.17742336852233828Clontech Universal Primer Mix Long (95% over 23bp)
GGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCG240190.17709894396118703Clontech Universal Primer Mix Long (95% over 23bp)
AGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTA239330.17646484140984592No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG235850.17389893806255863Clontech Universal Primer Mix Long (95% over 22bp)
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA231660.17080953144614092No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGG223080.16448325250369125Clontech Universal Primer Mix Long (100% over 23bp)
TAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCA221230.1631191946897598Clontech Universal Primer Mix Long (100% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCT217010.16000766821689993Clontech Universal Primer Mix Long (95% over 24bp)
GACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACG209070.15415327954521574Clontech Universal Primer Mix Long (95% over 24bp)
AGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGG208230.15353392356483606Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGG208120.15345281742454825Clontech Universal Primer Mix Long (100% over 23bp)
CTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGT207650.15310627300695487No Hit
GTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG205580.15158000291244778Clontech Universal Primer Mix Long (95% over 23bp)
TATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGA205060.1511965920674508Clontech Universal Primer Mix Long (100% over 23bp)
CGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCC203220.14983990753900006Clontech Universal Primer Mix Long (95% over 24bp)
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC200510.1478417471737275Clontech Universal Primer Mix Long (95% over 23bp)
GGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAG200330.1477090280350747Clontech Universal Primer Mix Long (95% over 23bp)
GCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTAT190550.14049795483493976Clontech Universal Primer Mix Short (100% over 22bp)
GATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTC188540.13901592445331698Clontech Universal Primer Mix Long (100% over 23bp)
AGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAG185280.13661223338660533Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG179820.13258641951413735Clontech Universal Primer Mix Long (95% over 24bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCC179270.1321808888126983Clontech Universal Primer Mix Long (100% over 23bp)
TAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAG177800.13109701584703382No Hit
TGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTA175540.12943065332839324Clontech Universal Primer Mix Long (100% over 23bp)
AGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCT174060.12833940707724809Clontech Universal Primer Mix Long (100% over 23bp)
ACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAG170870.1259873290089014No Hit
TGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGC161620.11916703993924413Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTCTAATA156920.11570159576331017Clontech Universal Primer Mix Long (95% over 23bp)
CAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTG151620.11179375445853357Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCT149700.11037808364623714Clontech Universal Primer Mix Long (95% over 24bp)
ACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGC147090.10845365613577167Clontech Universal Primer Mix Long (100% over 23bp)
TTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATC144560.10658821490915191Clontech Universal Primer Mix Long (100% over 23bp)
TATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGG144550.1065808416236712Clontech Universal Primer Mix Short (100% over 21bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGC143200.10558544808377526Clontech Universal Primer Mix Long (95% over 24bp)
GGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGC142070.10475226682445497Clontech Universal Primer Mix Long (95% over 23bp)
TAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCC141500.10433198955205447Clontech Universal Primer Mix Long (100% over 23bp)
GTATTAGGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG137360.10127944936304031Clontech Universal Primer Mix Long (100% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)