FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n01_DAM-GR-bZIP1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n01_DAM-GR-bZIP1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13028867
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG4213863.2342489949433055Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA2361581.8125751072599023Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACT2251791.7283083786180333Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC1837451.4102914704709166Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA1270670.9752728307073824Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAG1081110.8297805173696224Clontech Universal Primer Mix Long (100% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCCTATAGTGAGTCGTATTAGAGATCGG1069310.8207237052922559Clontech Universal Primer Mix Short (100% over 22bp)
AGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC989460.7594367184805861No Hit
ATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAG988030.7583391556610409No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAATACGA869330.6672337663743133Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTA763160.5857454834714332Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGC737170.5657974711078101Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTAATACG713680.5477682748622732Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA674740.5178807950069642Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGAGATCGGAAG670750.5148183644824987Clontech Universal Primer Mix Long (95% over 23bp)
CACTATAGGGCAGCGTGGTCGCGGCCCTATAGTGAGTCGTATTAGAGATC634180.4867499223071354Clontech Universal Primer Mix Short (100% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATACGACTC594460.45626377182298355Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCG585950.44973212175701843Clontech Universal Primer Mix Long (100% over 23bp)
CTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAG568130.43605480046730083Clontech Universal Primer Mix Long (95% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAATACGAC557050.4275506074319433Clontech Universal Primer Mix Long (95% over 23bp)
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT538420.4132515897199656No Hit
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTAT500820.3843925953039508No Hit
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG495390.38022492669546787Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGCTAATACGA476690.36587218213218387Clontech Universal Primer Mix Long (95% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCCTATAGTGAGTCGTATTAGAGA475080.36463646455213644Clontech Universal Primer Mix Short (100% over 22bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGC459080.35235604139638543Clontech Universal Primer Mix Long (95% over 21bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC435900.33456477834949117Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT396040.3039711741627265Clontech Universal Primer Mix Long (95% over 24bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCC367320.28192781459815347Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC364140.279487080495948Clontech Universal Primer Mix Long (95% over 23bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA351750.2699774278147133No Hit
CTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTG347840.26697639940602663No Hit
CTAATACGACTCACTATAGGGCAGCGACCACGCTGCCCTATAGAGATCGG343720.26381419044342075Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGA331820.254680625721331Clontech Universal Primer Mix Long (95% over 21bp)
CCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAG328560.2521784895033467Clontech Universal Primer Mix Short (100% over 21bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC325820.2500754670379243Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG316330.24279164105366954Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA315440.24210854251563085Clontech Universal Primer Mix Long (95% over 23bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCT312920.24017437586860008Clontech Universal Primer Mix Long (95% over 24bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG308120.23649024892187478Clontech Universal Primer Mix Long (95% over 23bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTG305920.23480169073795903Clontech Universal Primer Mix Long (95% over 24bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACG293860.2255453217843117Clontech Universal Primer Mix Long (100% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACA291460.22370325831094906Clontech Universal Primer Mix Long (100% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACTCACTATAGG286630.21999610557080673No Hit
ACTATAGGGCAGCGTGGTCGCGGCCCTATAGTGAGTCGTATTAGAGATCG283620.21768585096463106Clontech Universal Primer Mix Long (95% over 23bp)
TAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT273120.20962682326866947No Hit
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC267780.2055282320404376Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGAC266780.20476070559320314Clontech Universal Primer Mix Long (95% over 21bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG258640.19851304031271483Clontech Universal Primer Mix Long (95% over 24bp)
GTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCG255670.19623348676442853No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTATAGGG249380.19140574541132394Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCG236130.18123601998546765Clontech Universal Primer Mix Long (95% over 22bp)
CTAATACGACTCACTATAGGGCAGCGACCACGCTGCCCTATAGTGAGATC226580.1739061424143788Clontech Universal Primer Mix Long (95% over 23bp)
CAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGG225520.1730925643803103Clontech Universal Primer Mix Long (95% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGG213550.16390527280691405Clontech Universal Primer Mix Short (100% over 21bp)
ATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCG209360.16068933699300178No Hit
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAG206190.1582562781552686Clontech Universal Primer Mix Long (100% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA199900.15342853680216398No Hit
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCAC197830.1518397570563887Clontech Universal Primer Mix Long (95% over 24bp)
CCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCC194060.14894618235031487Clontech Universal Primer Mix Long (95% over 22bp)
CTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCACGCTGCCCTAT193550.1485547438622253Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGA193460.1484856664819742Clontech Universal Primer Mix Long (95% over 24bp)
CCCTATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAGTGA188030.14431799787349123No Hit
CTAATACGACTCACTATAGGGCAGCGACCACGCTGCCCTATAGTGAGAGA181570.13935977702435676Clontech Universal Primer Mix Long (95% over 23bp)
ATAGTGAGTCGTATTATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT181180.1390604417099353No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC178010.13662738287220216Clontech Universal Primer Mix Long (95% over 24bp)
CTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACT172540.1324290132058298Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT172360.1322908584453276No Hit
ATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA170710.13102443980739079Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGC161480.12394017069941692Clontech Universal Primer Mix Long (100% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACG161460.12392482017047222Clontech Universal Primer Mix Long (100% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCA159620.12251257150756087Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCC156160.1198569300001297Clontech Universal Primer Mix Long (95% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGAGATCGGAAGAGCACACG155740.11953456889229125Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGGCTAATACG155170.11909707881736763Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGAGATCGGAAGAGCACA155090.11903567670158886Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGAGATCGGAAGAGCACACGTCT150130.11522874552330606Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATCCTCGGCCGCGACCACGCTGCCCTATAGT148560.114023729001148Clontech Universal Primer Mix Long (95% over 21bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACTCA148150.11370904315778187Clontech Universal Primer Mix Long (95% over 21bp)
CAGCGTGGTCGCGGCCGAGGAATACGACTCACTATAGGGCAGCGTGGTCG144840.1111685306174359Clontech Universal Primer Mix Long (95% over 21bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGTACGACT143690.11028587520311628Clontech Universal Primer Mix Long (95% over 23bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC142980.10974093142557984No Hit
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA142390.10928809082171151Clontech Universal Primer Mix Short (100% over 21bp)
AGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG139450.10703156306684228Clontech Universal Primer Mix Long (100% over 23bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT136330.10463688055147083Clontech Universal Primer Mix Long (95% over 24bp)
CTGCCCTATAGTGAGTCGTATTAATCCTCGGCCGCGACCACGCTGCCCTA133700.10261828599524424Clontech Universal Primer Mix Long (95% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACTCACTATAGGGCAG132970.10205799168876312Clontech Universal Primer Mix Long (95% over 21bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCAC131580.10099112992710724No Hit
CTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGT130660.10028500559565157No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)