FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n01_DAM-GR-EV-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n01_DAM-GR-EV-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14536792
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC7268284.999920202476585Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG2658711.8289523575765547Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT1871891.2876912595296128Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC1531421.0534786492095367Clontech Universal Primer Mix Long (95% over 23bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA1407570.9682810347702575No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG1380410.9495974077361772Clontech Universal Primer Mix Long (100% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC1308760.9003086788336794Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC1278480.8794787735836078Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC984270.6770888652737138Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC876860.6032004860494667Clontech Universal Primer Mix Long (95% over 23bp)
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT770790.5302339058026008No Hit
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT738310.5078905992463811Clontech Universal Primer Mix Long (95% over 24bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC709640.48816822858853587No Hit
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA679220.46724201598261844Clontech Universal Primer Mix Long (95% over 21bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG658830.4532155375133661Clontech Universal Primer Mix Long (95% over 23bp)
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG611220.4204641574289568Clontech Universal Primer Mix Long (100% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC575200.3956856505892084Clontech Universal Primer Mix Long (100% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA541040.37218665576283955No Hit
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC513450.353207227564376Clontech Universal Primer Mix Long (100% over 23bp)
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG500170.3440717869527197Clontech Universal Primer Mix Long (95% over 23bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG495250.34068727130442533Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA453740.3121321402961534Clontech Universal Primer Mix Long (95% over 23bp)
TAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAG440250.30285223865072847No Hit
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA430120.29588371354560206Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA417480.2871885351320979Clontech Universal Primer Mix Long (100% over 22bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA377830.2599129161372055No Hit
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC377170.25945889574536113Clontech Universal Primer Mix Long (95% over 24bp)
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA372330.2561294128718358Clontech Universal Primer Mix Short (100% over 21bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTCTAATA361450.24864495550324994Clontech Universal Primer Mix Long (95% over 23bp)
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG358180.24639549083456652Clontech Universal Primer Mix Long (100% over 23bp)
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC348100.2394613612136708Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG327430.2252422680327269Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTCTAATA300880.20697826590626048Clontech Universal Primer Mix Long (95% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG288860.19870959149721618No Hit
CTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCT271290.18662301833857153Clontech Universal Primer Mix Long (95% over 24bp)
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG262750.18074826963197932Clontech Universal Primer Mix Long (95% over 24bp)
CTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCT257750.17730872120891597Clontech Universal Primer Mix Long (95% over 24bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT254290.17492855370015611No Hit
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG250050.17201181663739842Clontech Universal Primer Mix Long (95% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC249700.17177104824778397No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG247960.1705740853965579Clontech Universal Primer Mix Long (100% over 23bp)
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC242720.1669694386491875Clontech Universal Primer Mix Long (95% over 23bp)
CGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTC239580.16480940223950374Clontech Universal Primer Mix Long (95% over 23bp)
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT237930.16367435125989283Clontech Universal Primer Mix Long (95% over 23bp)
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC233320.16050308761382842Clontech Universal Primer Mix Short (100% over 22bp)
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA222770.15324564044116473No Hit
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCC222520.15307366302001157Clontech Universal Primer Mix Long (100% over 23bp)
GTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAT218540.15033578247525314No Hit
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA214260.1473915290251109Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA212930.14647660914457605Clontech Universal Primer Mix Long (100% over 23bp)
CTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGT198920.13683899446315254No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG193420.13305549119778284Clontech Universal Primer Mix Long (95% over 22bp)
ATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCTATAGTGA193000.1327665691302455No Hit
ATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAT191670.13185164924971068Clontech Universal Primer Mix Long (100% over 23bp)
ATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCTATAGTGA189950.13066844459217686No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGG187700.12912064780179835Clontech Universal Primer Mix Long (100% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCT180230.12398196245774169Clontech Universal Primer Mix Long (95% over 24bp)
GGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCG180080.12387877600504982Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGC178080.12250295663582447Clontech Universal Primer Mix Long (95% over 24bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCC176790.12161555314267411Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGG176700.12155364127105898Clontech Universal Primer Mix Long (100% over 23bp)
TAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCA176510.12142293843098258Clontech Universal Primer Mix Long (100% over 23bp)
GCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGA175930.12102395081390721Clontech Universal Primer Mix Long (100% over 23bp)
GAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGT175260.12056305132521675No Hit
GGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCG172140.11841677310922519Clontech Universal Primer Mix Long (95% over 24bp)
TCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGC169440.11655941696077099Clontech Universal Primer Mix Long (95% over 23bp)
CAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG167210.11502537836408473Clontech Universal Primer Mix Long (95% over 22bp)
CCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAG166230.11435122687316432No Hit
AGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTA162950.11209488310763475No Hit
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC161360.11100110670910061Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCC161010.11076033831948617Clontech Universal Primer Mix Long (100% over 23bp)
CGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCC155570.10701810963519325Clontech Universal Primer Mix Long (95% over 24bp)
CAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTG155190.10675670395504042Clontech Universal Primer Mix Long (95% over 23bp)
GTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG146900.10105393266960137Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATACGA146340.1006687032462183Clontech Universal Primer Mix Long (95% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)