FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n01_DAM-GR-EV-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n01_DAM-GR-EV-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13575398
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC5941114.376379977957185Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG2711031.9970169567035896Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT1909431.406537031179491Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG1831771.349330605261076Clontech Universal Primer Mix Long (100% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC1394031.026879653915119Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC1292030.9517437352481305Clontech Universal Primer Mix Long (95% over 24bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA1059210.780242317757461No Hit
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG1045130.7698706144748021Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC937230.6903885985515857Clontech Universal Primer Mix Long (95% over 23bp)
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA838590.6177277454406861Clontech Universal Primer Mix Long (95% over 23bp)
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG783350.5770364890959366Clontech Universal Primer Mix Long (100% over 23bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT780850.5751949224619418Clontech Universal Primer Mix Long (95% over 24bp)
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG741880.5464885817712306Clontech Universal Primer Mix Long (95% over 23bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG727070.5355791410314453Clontech Universal Primer Mix Long (95% over 23bp)
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG691650.5094878249610066Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC676620.49841632635742983Clontech Universal Primer Mix Long (95% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC649740.4786158019087175Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA614720.4528191364997181Clontech Universal Primer Mix Long (95% over 21bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC581380.42826000386876323Clontech Universal Primer Mix Long (100% over 23bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC567590.4181019223156478No Hit
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA518550.3819777512232054No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA511940.37710864904292307Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA482190.35519400609838475Clontech Universal Primer Mix Long (100% over 22bp)
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT474550.34956617846489657No Hit
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC434780.320270536451307Clontech Universal Primer Mix Long (95% over 24bp)
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC432860.31885621327639896Clontech Universal Primer Mix Long (95% over 23bp)
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA419410.30894858478550685Clontech Universal Primer Mix Short (100% over 21bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC409220.30144235918534396Clontech Universal Primer Mix Long (95% over 23bp)
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG392560.2891701591364025Clontech Universal Primer Mix Long (100% over 23bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA392050.28879447954306753No Hit
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC388660.28629731518737056Clontech Universal Primer Mix Long (100% over 23bp)
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT350470.25816554328646574Clontech Universal Primer Mix Long (95% over 23bp)
GGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAG344030.25342166763729507Clontech Universal Primer Mix Long (95% over 23bp)
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA332020.24457478152758394Clontech Universal Primer Mix Long (95% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG329900.24301313302195635No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG302200.22260857471729373Clontech Universal Primer Mix Long (100% over 23bp)
CGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTC276930.20399401918087412Clontech Universal Primer Mix Long (95% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC274020.20185043561890414No Hit
GTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAT272320.20059817030778768No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTCTAATA270890.1995447941931426Clontech Universal Primer Mix Long (95% over 23bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT269760.198712406074577No Hit
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC265330.19544914999913818Clontech Universal Primer Mix Short (100% over 22bp)
ATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAT263210.19388750149351053Clontech Universal Primer Mix Long (100% over 23bp)
TAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAG259200.19093362861258284No Hit
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA256030.18859852212067743No Hit
CTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCT252880.18627814816184396Clontech Universal Primer Mix Long (95% over 24bp)
AGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTA237350.17483833623146813No Hit
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG230280.1696303857905308Clontech Universal Primer Mix Long (95% over 22bp)
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC228020.16796560955339945Clontech Universal Primer Mix Long (95% over 23bp)
GGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCG220480.1624114445852711Clontech Universal Primer Mix Long (95% over 24bp)
GGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCG218200.1607319358150678Clontech Universal Primer Mix Long (95% over 23bp)
CTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGT217310.16007633809336566No Hit
GAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGT213510.15727715680969354No Hit
CCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAG213350.15715929654511787No Hit
GCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGA212990.1568941109498226Clontech Universal Primer Mix Long (100% over 23bp)
TCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGC208180.15335093674601658Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCC206340.15199554370339638Clontech Universal Primer Mix Long (100% over 23bp)
GATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTC203410.14983722760835447Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGC202540.14919636241972428Clontech Universal Primer Mix Long (95% over 24bp)
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG196090.14444512050401762Clontech Universal Primer Mix Long (95% over 24bp)
TATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGA192960.14213947907825614Clontech Universal Primer Mix Long (100% over 23bp)
TAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCA192390.1417196018857053Clontech Universal Primer Mix Long (100% over 23bp)
AGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGG189380.13950235565837554Clontech Universal Primer Mix Long (95% over 23bp)
GTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG185670.13676947077352725Clontech Universal Primer Mix Long (95% over 23bp)
CGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCC185290.13648955264516Clontech Universal Primer Mix Long (95% over 24bp)
AGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAG183990.13553193799548272Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA170760.12578636736838214Clontech Universal Primer Mix Long (100% over 23bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGG169670.12498344431596038Clontech Universal Primer Mix Long (95% over 24bp)
AGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCT168560.12416578873046667Clontech Universal Primer Mix Long (100% over 23bp)
GTATTAGGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG164310.12103512545267549Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTCTAATA164230.12097619532038767Clontech Universal Primer Mix Long (95% over 23bp)
ACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAG161270.11879578042573781No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGG158410.11668902819644772Clontech Universal Primer Mix Long (100% over 23bp)
GACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACG156470.11525997248846775Clontech Universal Primer Mix Long (95% over 24bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCG148470.10936695925968432Clontech Universal Primer Mix Long (95% over 24bp)
GCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTAT146420.10785687461980857Clontech Universal Primer Mix Short (100% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG143310.10556596572711902Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGG140870.10376859669234006Clontech Universal Primer Mix Long (100% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCT139760.10295094110684636Clontech Universal Primer Mix Long (95% over 24bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)