FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34209048
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3036850.080.356282
GATCGGA3060450.080.1374051
TCGGAAG3000300.079.981063
CGGAAGA3001850.079.286614
GAGCACA2943450.076.2244649
AGAGCAC2989150.075.709838
GAAGAGC3094650.074.708676
AAGAGCA3191250.072.012727
GGAAGAG3324050.070.388025
ACACGTC2617850.041.39026612-13
CACGTCT2624100.041.29897714-15
CACACGT2677000.041.1332212-13
CGTCTGA2622000.040.9415416-17
ACGTCTG2618850.040.68341414-15
GCACACG2701900.040.68279610-11
AGCACAC2936150.038.3911910-11
TCTGAAC2821550.037.7046118-19
GAACTCC2735500.037.6837220-21
GTCTGAA2841450.037.38463216-17
CTGAACT2812800.037.284618-19
Produced by FastQC (version 0.11.5)