FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68197605
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC28345024.156307248619655TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA4497800.064.9075249
ATCGGAA4599500.064.2731862
AGAGCAC4621150.063.3250058
TCGGAAG4678850.063.089383
CGGAAGA4679300.063.014924
GATCGGA4704450.062.8640331
AAGAGCA4724400.062.1255047
GAAGAGC4841700.060.6961026
GGAAGAG5329250.055.3047265
CTCGTAT3172850.044.745442-43
TCGTATG3157150.044.2818642-43
TATGCCG3301250.044.17375646-47
CGTATGC3300700.044.1736344-45
ATCTCGT3228350.043.95193540-41
TATCTCG3192850.043.67466738-39
TCTCGTA3205400.043.62349740-41
ATATCTC3283450.043.14803338-39
ATGCCGT3323350.043.1046246-47
CAATATC3402300.042.70320536-37
CCGTCTT3413600.042.69371850-51
Produced by FastQC (version 0.11.5)