FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48412559
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC28621975.9120960740786295TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGTATGC665810.13752836324970966TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3821900.080.619352
GATCGGA3843100.080.253081
TCGGAAG3824550.080.169083
CGGAAGA3824250.079.8699954
GAGCACA3925750.076.546259
AGAGCAC3986800.075.5349358
GAAGAGC4023050.075.280836
AAGAGCA4187500.072.234227
GGAAGAG4336350.070.1310655
CTCGTAT3178150.045.39225442-43
CGTATGC3269150.045.08188644-45
ATCTCGT3231650.044.95013440-41
TATGCCG3283400.044.8785746-47
CCGTCTT3279950.044.81893550-51
TGCCGTC3301250.044.70736348-49
TCGTATG3152700.044.66486442-43
TCTCGTA3191900.044.43995340-41
ACGGTAG3425000.044.1853132-33
ATGCCGT3264800.044.09253746-47
TCACGGT3465950.043.6893130-31
Produced by FastQC (version 0.11.5)