FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51986053
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC48416149.313294086781314TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATGC1293510.24881865911228152TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5778450.090.477211
ATCGGAA5776300.090.3792
TCGGAAG5798600.089.720933
CGGAAGA5822350.089.0329064
GAGCACA5983950.085.170279
GAAGAGC6055100.084.9689256
AGAGCAC6017800.084.888528
AAGAGCA6230400.082.4207157
GGAAGAG6367650.081.099675
CTCGTAT5339850.046.01743342-43
ATCTCGT5384150.045.7434640-41
CGTATGC5558550.045.67444-45
TATGCCG5573150.045.55429546-47
CCGTCTT5562450.045.48045350-51
ACGAGTG5643750.045.46312732-33
TGCCGTC5588950.045.45887848-49
CGTCTGA5642050.045.32122816-17
TCACGAG5677900.045.26206230-31
AGTCACG5698050.045.08407628-29
TCGTATG5328650.044.9513342-43
Produced by FastQC (version 0.11.5)