FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50701804
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC952381318.783972657067586TruSeq Adapter, Index 22 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGTATGC1951050.38480879299679355TruSeq Adapter, Index 22 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10791500.093.651971
ATCGGAA10799400.093.514552
TCGGAAG10822250.093.0399253
CGGAAGA10900000.092.11324
GAAGAGC11135800.089.6341256
GAGCACA11083400.089.4939659
AGAGCAC11125500.089.321388
AAGAGCA11314000.088.12317
GGAAGAG11441400.087.475065
CGTCTGA10814200.046.1556816-17
CTCGTAT10327450.046.14322742-43
CGATCTC10442000.046.0901738-39
CACGTCT10840900.046.03613714-15
ATCTCGT10409600.046.01162740-41
CACACGT10862300.045.96095712-13
CGTACGA10751300.045.92577434-35
TACGATC10770650.045.90173336-37
CGTATGC10590650.045.8658344-45
ACCGTAC10819600.045.85671632-33
TATGCCG10603200.045.79496846-47
Produced by FastQC (version 0.11.5)