FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49561469
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC45801899.24143108026116TruSeq Adapter, Index 21 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCGCGTATGC1120290.22604051546575424TruSeq Adapter, Index 21 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5279050.090.7303541
ATCGGAA5282900.090.599262
TCGGAAG5315750.089.9256743
CGGAAGA5345800.089.22794
GAGCACA5533050.085.101429
AGAGCAC5550050.084.989678
GAAGAGC5593100.084.721236
AAGAGCA5771950.082.034837
GGAAGAG5872750.080.920795
CTCGTAT4957300.046.26418742-43
CGATCTC4972200.046.05993738-39
ATCTCGT4987250.046.00540-41
CGTATGC5132500.045.9907244-45
TTCGATC5128600.045.94453436-37
TATGCCG5149350.045.86686346-47
GTTTCGA5131150.045.8651234-35
TGCCGTC5157850.045.7689748-49
CCGTCTT5147450.045.72304550-51
TCGTATG4951300.045.6986142-43
TCTCGTA4959900.045.6438640-41
Produced by FastQC (version 0.11.5)