FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47729024
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC15679763.285162504056232TruSeq Adapter, Index 20 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2143700.077.306252
GATCGGA2162450.076.679611
TCGGAAG2192800.075.454443
CGGAAGA2210650.074.609164
GAGCACA2364550.068.86259
AGAGCAC2459250.066.378818
GAAGAGC2493000.065.826
AAGAGCA2666250.061.5321437
GGAAGAG2828650.058.2633065
CTCGTAT1740000.045.2364742-43
CGTATGC1816100.044.7336444-45
TCGTATG1736350.044.5560242-43
TATGCCG1830550.044.41054546-47
ATCTCGT1775050.044.35271540-41
TCTCGTA1750950.044.20495640-41
ATGCCGT1826750.043.6045546-47
CCGTCTT1864450.043.49123850-51
TGCCGTC1871000.043.44685448-49
CATCTCG1805600.042.79740538-39
AGTCACG1916300.042.5974228-29
Produced by FastQC (version 0.11.5)