FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51349002
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGC518472710.097035576270791TruSeq Adapter, Index 19 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGTATGC1061850.20679077657633932TruSeq Adapter, Index 19 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA6372550.086.358572
GATCGGA6394150.086.165431
TCGGAAG6418700.085.572173
CGGAAGA6445200.084.9941564
GAGCACA6583950.082.201579
GAAGAGC6709750.081.229466
AGAGCAC6686050.081.183578
AAGAGCA6851200.079.4530947
GGAAGAG7089200.077.095535
CTCGTAT5751850.045.29817242-43
TCGTATG5737600.045.13168342-43
TCTCGTA5768950.045.0034340-41
ATCTCGT5802650.044.97986640-41
CGTATGC5941300.044.90184444-45
TATGCCG5952600.044.8217946-47
AATCTCG5804350.044.7652538-39
ATGCCGT5935400.044.6899846-47
CCGTCTT5946700.044.66972750-51
TGCCGTC5982150.044.56294348-49
AGTCACG6123850.044.2871228-29
Produced by FastQC (version 0.11.5)