FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51902084
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC12633622.4341257665106473TruSeq Adapter, Index 18 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA2305750.057.585924
ATCGGAA2318100.057.5740242
TCGGAAG2318650.057.393093
GATCGGA2358250.056.621521
GAGCACA2390300.054.938349
AGAGCAC2503050.052.624938
GAAGAGC2573300.051.4454156
AAGAGCA2774750.047.7427147
GGAAGAG2943050.045.1466945
CTCGTAT1430800.044.29759642-43
TCGTATG1424450.043.87483242-43
CGTATGC1499500.043.658644-45
TCTCGTA1440900.043.410240-41
TATGCCG1510150.043.36664646-47
ATCTCGT1472150.043.0985340-41
ATGCCGT1506150.042.76119646-47
TGCCGTC1535750.042.6379348-49
CCGTCTT1531350.042.5822150-51
CGCATCT1478450.042.12969636-37
ACGTCCG1558750.042.11605532-33
Produced by FastQC (version 0.11.5)