FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47592989
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC34232247.192706471955354TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCTCGTATGC17146553.602747034862635TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGTATGC695870.1462127121286709TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA6487550.084.072682
GATCGGA6501300.083.951321
TCGGAAG6484650.083.838463
CGGAAGA6500450.083.3950044
GAGCACA6567500.081.4983759
AGAGCAC6663050.080.486498
GAAGAGC6697250.080.464276
AAGAGCA6897400.078.030557
GGAAGAG6995100.077.247945
CTCGTAT5564200.046.23608442-43
CGTATGC5718600.046.03305444-45
ATCTCGT5612050.045.96157540-41
TATGCCG5790200.045.88046346-47
CCGTCTT5773550.045.78226550-51
TGCCGTC5800850.045.77229348-49
TCGTATG5549550.045.5116442-43
ACGTCGA1947800.045.43984232-33
TCTCGTA5576050.045.42946640-41
GATCTCG5627300.045.16641238-39
ACGTAGA3993450.045.08895532-33
Produced by FastQC (version 0.11.5)