FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53131716
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC23656264.452380194157478TruSeq Adapter, Index 16 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCGCGTATGC733910.13813030243555469TruSeq Adapter, Index 16 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2968450.084.731252
GATCGGA2971300.084.7107241
TCGGAAG3035050.082.7727053
CGGAAGA3070000.081.6636354
GAGCACA3326500.074.497859
GAAGAGC3462400.072.1081166
AGAGCAC3449200.072.021228
AAGAGCA3731550.066.8687747
GGAAGAG3816200.065.628345
CTCGTAT2627900.045.1153842-43
ATCTCGT2659650.044.59187740-41
TCGTATG2626150.044.52233542-43
CGTATGC2769700.044.51373744-45
TCTCGTA2632400.044.4147240-41
TATGCCG2781450.044.359646-47
ACCCGTC2812200.043.87177732-33
TGCCGTC2816050.043.8476448-49
CCGTCTT2810350.043.8081650-51
ATGCCGT2779150.043.69298646-47
CATCTCG2686600.043.48900238-39
Produced by FastQC (version 0.11.5)