FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54289339
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC18306533.3720303722983256TruSeq Adapter, Index 15 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2310150.084.670891
ATCGGAA2307050.084.543782
TCGGAAG2387850.081.254793
CGGAAGA2448450.078.927184
GAGCACA2743650.069.6880349
GAAGAGC2883200.066.757426
AGAGCAC2890100.066.337528
AAGAGCA3186100.060.4613727
GGAAGAG3262800.059.210575
CTCGTAT2085450.044.0533442-43
TCGTATG2075550.044.0357642-43
TCTCGTA2096950.043.73262840-41
CGTATGC2179250.043.2677244-45
AATCTCG2114100.043.21851738-39
ATCTCGT2133100.043.21527540-41
TATGCCG2182400.043.1935446-47
ATGCCGT2185600.043.02054646-47
TGCCGTC2223650.042.38712748-49
CCGTCTT2220200.042.3406750-51
GCCGTCT2235200.041.92690348-49
Produced by FastQC (version 0.11.5)