FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53812564
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC31072635.774233318449572TruSeq Adapter, Index 14 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCGCGTATGC887980.16501350874119286TruSeq Adapter, Index 14 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3761400.086.437121
ATCGGAA3769650.086.239172
TCGGAAG3827950.084.785013
CGGAAGA3854150.084.048264
GAGCACA4047500.079.2247549
AGAGCAC4169600.077.06428
GAAGAGC4194600.077.003366
AAGAGCA4452000.072.516877
GGAAGAG4511550.071.787495
CTCGTAT3371150.045.90027642-43
CGTATGC3523850.045.4309744-45
TATGCCG3527200.045.41580646-47
ATCTCGT3412500.045.36085540-41
TCGTATG3374350.045.16699242-43
TCTCGTA3377250.045.12124340-41
TGCCGTC3565650.044.93424648-49
CCGTCTT3560900.044.86612750-51
ATGCCGT3527750.044.61161846-47
CATCTCG3437200.044.30326538-39
CGTCTGA3638700.044.27931216-17
Produced by FastQC (version 0.11.5)