FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37420913
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC13350443.5676414415650415TruSeq Adapter, Index 13 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGATGC3561320.9516924400000608TruSeq Adapter, Index 13 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTTTGC1983690.5301019780035832TruSeq Adapter, Index 13 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATTC1816550.48543711373370285TruSeq Adapter, Index 13 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGTTGC575410.15376695913325256TruSeq Adapter, Index 13 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGATTC527710.14102007612695072TruSeq Adapter, Index 13 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGG462020.12346572089248598TruSeq Adapter, Index 13 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTGTGC407570.10891503368717914TruSeq Adapter, Index 13 (97% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2905100.089.3246151
ATCGGAA2891900.089.1139452
TCGGAAG2934000.087.572113
CGGAAGA2972400.086.262524
GAGCACA3151350.080.962529
GAAGAGC3217100.079.339476
AGAGCAC3209750.079.113568
AAGAGCA3393800.075.152247
GGAAGAG3451300.074.1840745
TCGTATG1498200.046.213942-43
ATGCCGT1580700.046.0516446-47
TCTCGTA1724200.045.9361840-41
CTCGTAT1711300.045.41916742-43
CGTATGC1504250.044.9070844-45
TATGCCG1498000.044.8713846-47
CGTCTTC2053250.044.81311450-51
GCCGTCT1805900.044.75542448-49
CGTCTGA2875750.044.73326516-17
ATCTCGT2098000.044.6808440-41
CCGTCTT2036400.044.6695950-51
Produced by FastQC (version 0.11.5)