FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35829525
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC12070473.368861295258589TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2304200.054.7830129
CGGAAGA2356250.054.156334
AGAGCAC2363750.053.4832428
TCGGAAG2409400.053.214673
ATCGGAA2454500.052.435312
GAAGAGC2459100.051.6614276
GATCGGA2500600.051.49091
AAGAGCA2574600.049.350727
GGAAGAG2750650.046.353175
CTCGTAT1358450.044.5796742-43
TCGTATG1358250.044.05817442-43
CGTATGC1424700.043.9653244-45
TCTCGTA1363900.043.86857240-41
CCGTCTT1430750.043.78343650-51
TGCCGTC1432750.043.75878548-49
ATCTCGT1386950.043.6789140-41
TATGCCG1437550.043.6157346-47
ATGCCGT1423000.043.46753346-47
GCCGTCT1434700.043.0719148-49
CGTCTTC1441150.042.86430750-51
Produced by FastQC (version 0.11.5)