FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41711266
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC26004276.234351649743741TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC671730.1610428223396528TruSeq Adapter, Index 10 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA3740500.071.769539
CGGAAGA3779550.071.69724
TCGGAAG3831850.070.881013
AGAGCAC3817500.070.4338158
ATCGGAA3875200.070.236162
GATCGGA3933200.069.216351
GAAGAGC3907650.069.105996
AAGAGCA4040650.066.7829747
GGAAGAG4230250.064.025865
CTCGTAT2841150.045.6195942-43
TCGTATG2837450.045.2999142-43
ATCTCGT2866650.045.22041340-41
TCTCGTA2844850.045.1879240-41
CGTATGC2959150.045.18395244-45
TGCCGTC2972100.044.9986448-49
TATGCCG2976550.044.9408346-47
CCGTCTT2968050.044.94001450-51
TATCTCG2860550.044.9091938-39
ATGCCGT2959450.044.79764646-47
GCCGTCT2970300.044.53896348-49
Produced by FastQC (version 0.11.5)