FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41463974
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC897819621.65300412353143TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGC1991050.4801879337470161TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG843500.20342960855609257TruSeq Adapter, Index 9 (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA10635000.087.82964
TCGGAAG10688350.087.70043
ATCGGAA10750250.087.558182
GATCGGA10800050.087.238141
GAGCACA10695750.086.161959
AGAGCAC10745750.085.9603968
GAAGAGC10817300.085.810666
AAGAGCA10946200.084.679027
GGAAGAG11090700.083.9205865
GATCGGG154450.076.955331
ATCGGGA160650.073.332872
TCGGGAA179950.063.7235763
CGGGAAG226950.049.4347044
TCGGAAA93600.047.654563
CTCGTAT9771850.046.37993642-43
ATCTCGT9805700.046.26920740-41
CGTATGC10100750.046.147744-45
CCGTCTT10083150.046.08435450-51
TATGCCG10118350.046.082946-47
TGCCGTC10120600.046.0663648-49
Produced by FastQC (version 0.11.5)