FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34101281
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC14108934.137360705012812TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC2058240.6035667692366161TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC1016850.29818527931546035TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC813820.23864792645179517TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA2591950.077.066494
TCGGAAG2608350.076.664473
ATCGGAA2616700.076.532022
GATCGGA2643750.075.817461
GAGCACA2665500.074.411969
AGAGCAC2708050.073.351518
GAAGAGC2752300.072.405716
AAGAGCA2891900.068.920047
GGAAGAG3011600.066.327695
TCGTATG1538800.046.30343642-43
ATGCCGT1609400.046.0857646-47
TCTCGTA1645100.045.9577340-41
GCCGTCT1704700.045.40337448-49
CTCGTAT1635450.045.28933742-43
CGTCTTC1797400.045.1085750-51
CGTATGC1562750.044.97494544-45
AATCTCG2074600.044.9125138-39
CCGTCTT1792550.044.8212150-51
TGCCGTC1757550.044.73941848-49
ATCTCGT1825750.044.72360640-41
Produced by FastQC (version 0.11.5)