FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33906596
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC22428066.614659873258878TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC524310.15463362939765465TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA3511350.065.980829
AGAGCAC3590850.064.666868
CGGAAGA3640950.064.230664
TCGGAAG3684450.063.578613
GAAGAGC3701800.063.0680666
ATCGGAA3721600.063.011812
GATCGGA3773400.062.1295741
AAGAGCA3792950.061.4945687
GGAAGAG3994250.058.5957035
CTCGTAT2455500.045.81731842-43
CGTATGC2542000.045.4925944-45
TATGCCG2553750.045.33592246-47
ATCTCGT2482100.045.31673440-41
CCGTCTT2549200.045.30338750-51
TGCCGTC2555050.045.29911448-49
TCGTATG2452700.045.24506442-43
TCTCGTA2461200.045.10424440-41
ATGCCGT2543500.044.81083746-47
GCCGTCT2554400.044.528748-49
CATCTCG2495050.044.4192638-39
Produced by FastQC (version 0.11.5)