FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37603613
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC25820946.866611460978497TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATGC544700.144853102280358TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATGC502000.13349781043645992TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA3763950.074.218954
TCGGAAG3795200.073.796043
ATCGGAA3809600.073.7018662
GAGCACA3759750.073.5425649
GATCGGA3850650.072.941841
AGAGCAC3830850.072.31658
GAAGAGC3908700.071.2344746
AAGAGCA4027750.069.0306247
GGAAGAG4207000.066.35765
CTCGTAT2823000.045.81093242-43
CGTATGC2857900.045.6195544-45
TCGTATG2775900.045.5187742-43
TATGCCG2866050.045.4349746-47
CCGTCTT2898000.045.41053450-51
ATCTCGT2893850.045.3417740-41
CGATCTC2959400.045.3183838-39
TGCCGTC2907850.045.29912648-49
TCTCGTA2836100.045.25345240-41
ATGCCGT2861000.045.17149746-47
CACGATC3072450.044.8458636-37
Produced by FastQC (version 0.11.5)