FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38168502
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC586947915.377808120423483TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGC1263990.33116049458791963TruSeq Adapter, Index 12 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGT448110.11740308802268426TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG7278600.084.08593
ATCGGAA7302100.083.961592
GATCGGA7335150.083.69861
CGGAAGA7319300.083.455474
GAGCACA7301450.082.944439
AGAGCAC7359450.082.399828
GAAGAGC7427800.081.8748866
AAGAGCA7550400.080.4522257
GGAAGAG7649650.079.606175
TCGTATG6358550.046.00994542-43
TCTCGTA6390400.045.93396840-41
AATCTCG6401150.045.7925138-39
ATGCCGT6561200.045.72308746-47
GCCGTCT6527250.045.71429448-49
CGTCTTC6525950.045.64011850-51
CTCGTAT6380500.045.41876642-43
GTATGCC6629800.045.3239544-45
ATCTCGT6418700.045.23785840-41
GTAATCT6479350.045.16325436-37
GCTTGAA6534300.045.14625558-59
Produced by FastQC (version 0.11.5)