FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41065733
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC15622903.804364091102429TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA2501450.065.241454
TCGGAAG2543450.064.334643
ATCGGAA2568150.063.8433762
GAGCACA2541350.063.601059
GATCGGA2591100.063.3066331
AGAGCAC2571100.062.990758
GAAGAGC2652250.061.3886386
AAGAGCA2800900.058.1115957
GGAAGAG2950900.055.306165
CTCGTAT1744850.044.7539642-43
TGCCGTC1810700.044.39934548-49
CGTATGC1812050.044.3497344-45
CCGTCTT1811750.044.28054450-51
ATCTCGT1770000.044.13297340-41
TCGTATG1741600.044.1242842-43
TATGCCG1828100.044.01683446-47
TCTCGTA1746750.044.01066640-41
ATGCCGT1806400.043.68705746-47
GCCGTCT1811850.043.46407748-49
CGTCTTC1817800.043.28000350-51
Produced by FastQC (version 0.11.5)