FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31283174
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC21764006.95709457103042TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC460510.14720692983391007TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2669300.085.820971
ATCGGAA2672300.085.656942
TCGGAAG2683700.085.00853
CGGAAGA2693150.084.472144
GAGCACA2859600.078.607189
AGAGCAC2884800.078.101628
GAAGAGC2900400.078.0331346
AAGAGCA3054550.074.0517
GGAAGAG3127000.072.641315
CTCGTAT2418700.045.07193842-43
ATCTCGT2437950.044.73271640-41
CGTATGC2509900.044.6560444-45
TGCCGTC2517650.044.54646748-49
CCGTCTT2514400.044.4850350-51
TCGTATG2414900.044.4839442-43
TATGCCG2524400.044.42996246-47
TCTCGTA2421700.044.37679340-41
GATCTCG2425400.044.13413238-39
ATGCCGT2512650.043.87589346-47
GCCGTCT2514900.043.74807748-49
Produced by FastQC (version 0.11.5)