FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43755212
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC699548715.987779924366496TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGC5882101.3443198492559012TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTC2463730.5630712062370993TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGC2450000.5599332943467398TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATGC1638210.3744033967884786TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCATCTCGTATGCC573890.13115923195618387TruSeq Adapter, Index 4 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9516400.092.672431
ATCGGAA9527750.092.512452
TCGGAAG9531450.092.148093
CGGAAGA9578750.091.401944
GAAGAGC9769650.089.011996
GAGCACA9730900.088.753639
AGAGCAC9759350.088.723168
AAGAGCA9972950.087.146217
GGAAGAG10042350.086.837615
TCGTATG7484800.046.4127342-43
ATGCCGT7731300.046.3096146-47
TCTCGTA7824650.046.19133840-41
CGTCTGA9489150.046.11129816-17
CTCGTAT7801950.046.03743442-43
CACGTCT9515750.045.981814-15
TCGGAAA85550.045.9213833
CGTATGC7657650.045.9121644-45
CCGTCTT8166100.045.8935450-51
GTATGCC7715650.045.8822344-45
TGCCGTC8111950.045.86189748-49
Produced by FastQC (version 0.11.5)