FastQCFastQC Report
Wed 5 Aug 2020
HNVFWDRXX_n01_GG102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFWDRXX_n01_GG102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42931875
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC432282910.069043106083766TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC1060450.24700761380675781TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4850750.092.775381
ATCGGAA4855200.092.533382
TCGGAAG4883000.091.852023
CGGAAGA4919150.090.9098364
GAGCACA5161400.085.966229
GAAGAGC5194250.085.69446
AGAGCAC5204350.085.302398
AAGAGCA5389900.082.535697
GGAAGAG5477950.081.4567265
TCGTATG4666800.045.95239342-43
TCTCGTA4671350.045.902240-41
CTCGTAT4668300.045.87047242-43
TATCTCG4667700.045.8424438-39
CGTCTGA4864500.045.62655316-17
ATCTCGT4691950.045.62170440-41
ATGCCGT4851850.045.58234446-47
ACACGTC4870750.045.51377512-13
CGTATGC4840050.045.5042744-45
CGATGTA4810400.045.49809334-35
CACCGAT4847950.045.44915430-31
Produced by FastQC (version 0.11.5)