FastQCFastQC Report
Fri 4 Sep 2020
HNVFNDRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFNDRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932068969
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT112027000.079.869411
AGCAGTG126545050.070.664342
GCAGTGG155553250.057.480593
CAGTGGT169713950.052.6415144
AGTGGTA176713300.050.5575875
GTGGTAT199605750.044.730956
TGGTATC201192950.044.3706677
GGTATCA206403850.043.2761738
GTATCAA208651800.042.8313149
CAACGCA210166300.024.13532612-13
ATCAACG210655400.024.07359310-11
ACGCAGA211383950.023.98060814-15
AGAGTAC211321900.023.8857818-19
AGTACAT211595100.023.78179620-21
TACATGG211395050.023.77930622-23
GCAGAGT212922350.023.75463916-17
CATGGGG79472750.022.9058324-25
CATGGGT57291600.022.3934624-25
TATCAAC208498350.021.47694810-11
TCAACGC209957650.021.33785612-13
Produced by FastQC (version 0.11.5)