FastQCFastQC Report
Fri 4 Sep 2020
HNVFNDRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFNDRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932068969
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATTGC695640787.463404567006886No Hit
CTCGTCAC649833616.971947694999383No Hit
ATTCCGAT618830436.639320163870835No Hit
GATCAGCA604488226.485445177394378No Hit
TGGTGTTT591744736.348722569692157No Hit
ACAACAGG586633586.293885962423881No Hit
GAAGGCTG560078706.008983440365989No Hit
CCCTAACA558892165.996253266532682No Hit
GCTGAATT544151405.838102308929029No Hit
TGAAGTAC530920105.696146075645181No Hit
CACTCGGA477523455.1232630404199195No Hit
ATGCTCCG474520205.0910417123864145No Hit
CCGGGTAT340175953.649686464350043No Hit
ATCATGCA318999013.4224828913921286No Hit
GGTTCCTC306841053.2920423295413883ABI Solid3 Adapter A (100% over 8bp)
TAACAAGG282813033.034250033057371No Hit
CAGCCCAC240210832.577178706611356No Hit
TTTGGGTA190585692.0447595225112574No Hit
GGCAATGG178577011.915920558878728No Hit
ACATTACT142438661.528198714230556No Hit
GGGGGGGG63888120.685444126184615No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)