FastQCFastQC Report
Fri 4 Sep 2020
HNVFNDRXX_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVFNDRXX_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932068969
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTGG1563350.016.0395471
CTCCTAT3971600.016.0278998
CTGTGGG1837900.015.4278481
CCGGACA1615300.015.3690431
TCCGGGA2167200.015.3229681
GGGCTAC1418450.015.2497011
TAACGAC2053800.015.176141
GCCCGAA1336250.015.1254091
CTGGACG1197400.015.0403181
TGGGGTC1226300.015.0283084
GGGATGA1530750.014.9008971
GGGGTCC1002550.014.87461955
AGGGCCT2229400.014.7740291
ATCCTCT4344700.014.7460243
CTGAAAG1713050.014.6741383
TCTCCTA4350800.014.6667757
GGGACCT1260100.014.5914911
TGGGCTG1330000.014.5825361
AATGGCT1757500.014.4229551
CCCTGAT1643550.014.3944591
Produced by FastQC (version 0.11.5)