FastQCFastQC Report
Sun 16 Aug 2020
HNVCKDRXX_l02_n02_B2_33___296.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVCKDRXX_l02_n02_B2_33___296.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390143
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230038.848226405484903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG589954.243808011118281No Hit
CCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGT83530.6008734353228409No Hit
TGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGG78160.5622443158725396No Hit
CCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGG78120.5619565756904146No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCT77450.5571369276398184No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA62090.44664469770376136No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG61850.44491825661101053No Hit
GCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC57620.41448973235127606No Hit
AAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCA44440.31967934234103973No Hit
GCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCC42660.3068749042364706No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA40170.28896307789918013No Hit
TGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCG39470.28392762471199007No Hit
TTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGC38750.2787483014337374No Hit
CAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACC38520.2770937953865178No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT38090.2740005884286724No Hit
TCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGC36040.2592539040947586No Hit
GGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGG35030.25198846449609863No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA33510.24105433757534295No Hit
GGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGT32980.23724178016218475No Hit
TAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGT32620.23465211852305842No Hit
GCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTC32320.23249406715711982No Hit
GGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACG31080.2235741215112402No Hit
GAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCG30620.220265109416801No Hit
GCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGC29790.2142945006377042No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC29050.20897130726838892No Hit
CCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGT28590.20566229517394974No Hit
GGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACC28380.2041516592177927No Hit
GTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGA27450.197461699983383No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC27400.19710202475572658No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA27250.19602299907275725No Hit
GCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGA26640.19163496129534877No Hit
GCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGG24140.17365119991252698No Hit
GCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCG23860.17163701863765096No Hit
GGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACT23400.16832800654321176No Hit
CTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAAC22740.1635802935381468No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA22570.16235739776411492No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC22180.1595519309883947No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC21740.1563867889850181No Hit
CCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAA21510.15473228293779848No Hit
GGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGG20490.1473949082936072No Hit
GCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAA20000.14387009106257415No Hit
GGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCG19990.14379815601704285No Hit
CAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCG19640.14128042942344782No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG18960.13638884632732026No Hit
CGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGG18630.13401498982478782No Hit
GGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGA17880.12861986140994128No Hit
CAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCG17720.1274689006814407No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC17380.12502310913337691No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC17150.12336860308615732No Hit
GCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTG17140.12329666804062604No Hit
GCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACG17100.1230089278585009No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16980.12214570731212546No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG16560.1191244353998114No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG16250.1168944489883415No Hit
GGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTT16170.1163189686240912No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC15910.11444865744027771No Hit
GGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGG15770.1134415668028397No Hit
TAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGCTGGGCCTCCCAACG15660.11265028130199556No Hit
GCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTT15560.11193093084668267No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC15220.10948513929861892No Hit
GAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGG14740.10603225711311715No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT14660.10545677674886685No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14540.1045935562024914No Hit
TCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGC14430.10380227070164724No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG14360.10329872538292822No Hit
GCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCT13930.10020551842508289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACTA1600.049.8399932
AATCCGT450.00964279248.329694
TCACTAG1801.8189894E-1244.3022163
AGACTCA5800.043.7971151
CTAGCAA1851.8189894E-1243.104866
AGAGAAC21800.042.2330554
CAGAGAG22350.041.5659681
ACTAGCA1953.6379788E-1240.8943565
CACTAGC2200.039.5424734
GCGGTAT750.001475758438.708311
AGAACCC23700.038.5413936
GAGAACC24250.037.966215
GACTCAG6600.037.345672
GAACCCA24800.036.8305747
GTTCACT2750.034.309641
GCACATA2008.962161E-932.622542
TAGCAAC2455.638867E-1132.5473947
CTCGTTT900.00360963632.2569241
TTCGGTC950.00470309530.5591931
GAGAGAA30750.030.1765863
Produced by FastQC (version 0.11.5)