FastQCFastQC Report
Sun 16 Aug 2020
HNVCKDRXX_l02_n01_B2_80_425.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVCKDRXX_l02_n01_B2_80_425.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26671636
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC2101440.7878931761066325No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC2090570.7838176855742932No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA977730.36658043773542803No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT940070.3524605689729719No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT822600.3084175263939565No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG801950.3006752191729071No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA734520.27539368038765977No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA733530.27502249955720753No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA732210.2745275917832712No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG730430.2738602161487207No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG720850.2702683854863646No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT701230.2629122563010383No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC696890.2612850595291567No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC687420.2577344711812954No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC672790.2522492433535011No Hit
TTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAG624640.23419635750877824No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG606410.22736138120661217No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC553460.20750883072939355No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT551330.20671022954872362No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC551020.2065940012078749No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA536740.20123999892620012No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC527690.19784688123368213No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC518300.19432628729636234No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT509930.19118812209344788No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC480420.18012393390491682No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC479090.17962527682966278No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT466550.1749236529772677No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG460950.172824044239356No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA453000.16984334969178494No Hit
CTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGC448230.1680549329632423No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT422990.1585916964373689No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC421080.15787557988568832No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG416470.15614715197822887No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG413000.15484614442098715No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC412250.1545649468221597No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG405260.15194418520108777No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA401320.15046696048191419No Hit
GCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCA398790.14951838724853622No Hit
GGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACC396780.14876477768367866No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA388360.14560786597417572No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC386900.1450604679817916No Hit
GCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCC380890.14280713788985422No Hit
CGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGG378380.14186606325911166No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG376500.14116119461138416No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT367650.13784306294522017No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT361920.13569471329017838No Hit
CAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCG361190.1354210142939863No Hit
GGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACA360540.13517730970833586No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA359630.1348361232884252No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC356690.13373382870102157No Hit
GCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTC356560.13368508778389146No Hit
GCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCA354690.13298396843748167No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA352940.13232784070688428No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA351670.13185167943953643No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA351160.13166046507233378No Hit
GTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCC338690.12698508632916256No Hit
GGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGG318560.11943774277663358No Hit
GGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCG314900.11806549849435558No Hit
GCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCA314570.11794177155087149No Hit
GGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCT313060.11737562705189887No Hit
GGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCG310180.11629582827240142No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA309260.1159508925511731No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC305030.11436493809378623No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC301860.1131764095760755TruSeq Adapter, Index 10 (100% over 50bp)
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG299490.11228782516378073No Hit
TCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTG298320.11184915690960989No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG298190.11180041599247979No Hit
CCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAA297790.11165044393977182No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT291850.10942335895705836No Hit
GAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCC285580.1070725470308608No Hit
CGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCC284360.10661513227010147No Hit
CAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGT283650.1063489318765448No Hit
GCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGC282280.10583527759601997No Hit
GCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCT281530.10555407999719253No Hit
GGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGA280940.10533287121944825No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA280830.10529162890495357No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC277330.10397937344375875No Hit
GCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTT276370.1036194405172596No Hit
CGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCA274650.10297456069061531No Hit
GGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCG274360.10286583095240201No Hit
TTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGG270280.10133611601478064No Hit
TGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAG269790.10115240025021337No Hit
GAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGC267130.10015508609970533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTCA297350.048.591862
CTCAGAG663900.048.041491
TCAGAGA666050.047.308942
CAGACTC310300.046.914751
GACTCAG338550.042.9143563
ACTCAGA484400.040.409864
CAGAGAG793550.039.595183
ACAGACT204850.038.5082552
GAGAGAA849300.037.0814255
AGAGAGA885450.035.903214
CAACTGT153850.035.6130941
AGAGAAC954750.032.9475866
GAGAACC970600.032.3273857
GTTCACT255250.032.240931
AGAACCC982850.032.034818
TTCACTA253050.031.5748442
AACCCAC993000.031.4378245
GAATCCA355500.031.2017924
ACTAGCA252300.031.005015
GAACCCA1051700.030.1243214
Produced by FastQC (version 0.11.5)