FastQCFastQC Report
Sun 16 Aug 2020
HNVCKDRXX_l02_n01_B2_71___381.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVCKDRXX_l02_n01_B2_71___381.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15234758
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC1060770.6962828027855775No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1054610.692239417258876No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT531480.3488601525537852No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT480920.3156728843346248No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA474420.3114063249314495No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA440750.2893055472230015No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG408630.2682221798337722No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA398640.26166480622796895No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC396150.2600303857796757No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG367120.24097527509134045No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA364170.23903891351605322No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC353890.23229118572149293No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT353440.2319958085320423No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC352260.23122126390192743No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG325750.21382026547451558No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG322590.21174606121081804No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC304370.19978656700684055No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA297560.19531652553982148No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT296640.19471264328583363No Hit
CTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGC269750.1770622152317746No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC266040.17462699440319301No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC262760.17247402288897534No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC260560.17102995662943907No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT260350.1708921139410288No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC257930.1693036410555389No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC256530.16838468979947038No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG249730.1639212122699947No Hit
TTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAG242610.15924768873913192No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG238860.15678621216037694No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT238380.1564711431582963No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG234770.1541015617051482No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC230230.15112153406046883No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA227470.14930988729850517No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA219420.14402591757611116No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT219370.14399309788839443No Hit
GTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCC219170.1438618191375275No Hit
GGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACA215610.14152505737209609No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC206920.1358209956469279No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC205430.13484296895296927No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA204120.13398309313479084No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG201320.1321451906226538No Hit
CAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCG198810.1304976422992738No Hit
CGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGG197970.12994627154563268No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC197330.1295261795428585No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA193730.12716316202725375No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA190250.12487891176216911No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA188160.12350704881560967No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT184750.12126874611332848No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT173260.11372678187602323No Hit
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG169920.11153442673654547No Hit
CAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGT168610.11067455091836706No Hit
GGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACC167310.109821239037732No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT165910.10890228778166347No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA165690.10875788115570985No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA165440.10859378271712619No Hit
CGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCC163180.10711033283232986No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG162730.10681495564287925No Hit
CGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCA162020.10634891607730165No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC159890.10495079738056882No Hit
GCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCA159370.1046094726283148No Hit
CGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGC158800.10423532818834404No Hit
GGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCT158690.10416312487536723No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC157670.10349360324594589No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA157110.10312602274351848No Hit
GGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCG153310.10063172647704675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGAGA358700.044.691762
AGACTCA161500.043.7223432
CTCAGAG374100.043.5309641
CAGACTC175250.040.747431
ACTCAGA244700.038.9001964
CAACTGT85550.038.3425981
GACTCAG190750.037.7023243
CAGAGAG432600.037.0027163
GAGAGAA435000.036.78195
ACAGACT112800.036.4141732
AGAGAGA453500.035.64914
TCACTAG138050.033.5046653
TTCACTA139600.033.3223572
AGAGAAC486050.032.7694176
CACTAGC142500.032.254884
GTTCACT147000.032.039851
GAGAACC506000.031.9828742
AACCCAC508500.031.5793955
AGAACCC511900.031.4687823
ATTAGGC107650.031.1130988
Produced by FastQC (version 0.11.5)