FastQCFastQC Report
Sun 16 Aug 2020
HNVCKDRXX_l02_n01_B2_31___286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVCKDRXX_l02_n01_B2_31___286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21518431
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC810420.37661667804683346No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC786470.36548668441486276No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT642400.29853477700116704No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA371860.17280999716010895No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT353660.16435213143560515No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG303040.14082811149195776No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG291570.13549779721393257No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA284150.13204959041855793No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC277880.12913580920467668No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG273200.12696092944694712No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA272470.12662168538217305No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA269940.12544594910288764No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC251670.11695555312559731No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT250250.11629565371192724No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC246750.11466914107259958No Hit
TTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAG238490.11083057124378633No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA238310.11074692202233517No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG226400.10521213186965164No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT215250.1000305273186507No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAG468400.028.229081
TCAGAGA454750.027.6880252
AACCCAC462250.026.5841545
AGAACCC489850.025.2927978
AGAGAAC492550.025.1104056
GAGAACC491800.025.045517
AGACTCA225950.024.9559382
ACTCAGA313900.024.4587754
TCACTAG132350.024.2118723
CACTAGC128550.024.0816274
GAACCCA528850.024.058844
GAGAGAA528450.023.9124225
CAGACTC247100.023.3779031
CAGAGAG546200.022.8035013
GACTCAG256150.022.557663
ACTAGCA140850.022.08165
AGAGAGA590700.021.8711074
TTCACTA158950.021.7325972
ACCCACC579800.021.4567576
GAATCCA233950.020.3907364
Produced by FastQC (version 0.11.5)