FastQCFastQC Report
Sun 16 Aug 2020
HNVCKDRXX_l01_n02_B1_63___70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVCKDRXX_l01_n02_B1_63___70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23666656
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT614880.25980856780104467No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA512030.21635080173557258No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC492420.20806488250811606No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC484810.20484938810113265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCAC370900.020.7962425
AGAACCC403750.019.3735393
GAGAACC420250.018.9746992
GAACCCA452200.018.564284
TCAGAGA428300.018.0256732
AGAGAAC418200.017.8893996
CACTAGC121900.017.6030564
GAATCCA251900.017.06584
TCACTAG125050.016.9857223
CTCAGAG500200.016.406481
AGACTCA219650.016.2376752
ACTCAGA301050.016.1819464
AGAATCC243600.016.0105063
ACTAGCA133600.015.9529495
AATCCAG280700.015.4439665
GCAGAAT276100.015.41387751
TTCACTA149650.015.258922
ACCCACC534400.014.8134526
CAGACTC257500.014.7504461
TAGCAAC140650.014.6893147
Produced by FastQC (version 0.11.5)