FastQCFastQC Report
Sun 16 Aug 2020
HNVCKDRXX_l01_n02_B1_53___108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVCKDRXX_l01_n02_B1_53___108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25359135
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC678640.26761165158038713No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC598800.23612792786504747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG354750.1398904181865825No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA351940.13878233622716232No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT294950.11630917221742776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAAC492300.021.882126
AGAACCC490450.021.5802278
AACCCAC450750.021.5511045
GAGAACC498350.021.3545887
CTCAGAG546100.020.984141
GAACCCA515200.020.7827249
TCAGAGA538750.020.5868572
AGACTCA259350.020.4043372
CAGACTC280650.019.3032381
GAGAGAA575450.018.896625
ACTCAGA353400.018.7901134
GACTCAG302400.017.8837033
GAATCCA258600.017.6608854
CAGAGAG620950.017.5586723
GCGGTAT26400.017.0547031
ACCCACC591800.016.8923386
AGAGAGA677250.016.6449244
AGAATCC254200.016.4551073
CACTAGC129900.016.295744
TCACTAG131400.016.2200583
Produced by FastQC (version 0.11.5)