FastQCFastQC Report
Sun 16 Aug 2020
HNVCKDRXX_l01_n01_B1_63___70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVCKDRXX_l01_n01_B1_63___70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23666656
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1006360.4252227268609473No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC482160.20372966928661151No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC468470.19794515963725504No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA438900.18545078780880578No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCAC365550.021.0021465
AGAACCC392400.019.8786858
AGAGAAC412650.018.9036166
TCAGAGA426000.018.8238622
GAGAACC417550.018.7703042
GAACCCA450950.018.2626744
CACTAGC115450.018.1475644
CTCAGAG486000.017.7243561
TCACTAG127150.017.446953
GAATCCA253000.016.9348534
ACTCAGA292150.016.7995344
ACTAGCA131600.016.3611975
AGACTCA210850.016.2795942
AGAATCC245600.015.9987263
ACCCACC507850.015.773986
GAGAGAA511450.015.6771245
TTCACTA153800.015.6180812
AATCCAG281400.014.9938565
CAGAGAG541950.014.8215973
GCAGAAT274750.014.4215181
Produced by FastQC (version 0.11.5)