FastQCFastQC Report
Sun 16 Aug 2020
HNVCKDRXX_l01_n01_B1_55___221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVCKDRXX_l01_n01_B1_55___221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21286647
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTAT857320.40275013721043057TruSeq Adapter, Index 16 (97% over 39bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC805810.37855186869026386No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC733390.34453054067181177No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT458400.2153462684846514No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCGCGTAT457860.21509258832544176TruSeq Adapter, Index 16 (97% over 39bp)
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA389700.18307251489631035No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT340310.15987017589007793No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA324100.1522550733330618No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG299800.14083946616862675No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC298440.14020056798987646No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG296340.13921403403739444No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA295970.13904021615052856No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC294360.13828387345362564No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA273680.1285688629120406No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA270180.12692463965790385No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG262500.12331674406025524No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC261350.12277649927675317No Hit
CTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGC247110.1160868595227797No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC245360.11526474789571133No Hit
TTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAG237520.11158168780644505No Hit
GCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCA236900.11129042540142654No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC233210.1095569443134938No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG230370.10822277458728No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT228360.10727852066133291No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG225470.10592086203148857No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC221110.10387262963490682No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC219150.10295186461259022No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT214740.10088014331237793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG37900.030.8271941
AGACTCA202750.030.172692
TCAGAGA435300.028.2404792
CAGACTC224550.027.9220941
GAATCCA293400.027.921784
CTCAGAG451450.027.7767471
AGAATCC286950.027.3619463
CACTAGC176150.027.1635234
GCAGAAT305750.026.86751
ACTCAGA298450.026.866334
AATCGGA42450.026.8391822
TCACTAG177400.026.8086553
GACTCAG239050.026.17263
AGAGAAC467950.025.8727046
TTCACTA193450.025.8087542
AACCCAC447450.025.7294655
ACTAGCA187350.025.5396545
AGAACCC477400.025.4960968
GTTCACT203050.025.4820231
CAACTGT144800.025.408951
Produced by FastQC (version 0.11.5)