FastQCFastQC Report
Sun 16 Aug 2020
HNVCKDRXX_l01_n01_B1_53___108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVCKDRXX_l01_n01_B1_53___108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25359135
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT778480.3069820796332367TruSeq Adapter, Index 14 (97% over 44bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC677640.26721731636351165No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC571640.22541778337470897No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT434790.17145300894529722TruSeq Adapter, Index 14 (97% over 45bp)
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA334610.13194850691870996No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT281430.11097776008527103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCAC421050.022.073415
AGAACCC471100.021.9435068
AGACTCA249400.021.3279152
GAGAACC486500.020.9963237
GAACCCA501050.020.9501519
AGAGAAC495100.020.8658926
CTCAGAG535850.020.7064271
ACTCAGA346300.020.3273984
TCAGAGA526350.020.2805122
CAGACTC271150.020.1256121
GAATCGG50150.019.3898641
GACTCAG292600.018.780623
CACTAGC126350.018.7623674
GAGAGAA568500.018.554435
TCACTAG133650.017.9545333
GAATCCA264800.017.93244
AGAATCC259250.017.2257083
ACTAGCA135450.017.180715
CAGAGAG617150.017.138773
ACCCACC558500.016.900636
Produced by FastQC (version 0.11.5)