FastQCFastQC Report
Sun 16 Aug 2020
HNVCKDRXX_l01_n01_B1_52___75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVCKDRXX_l01_n01_B1_52___75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9988106
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1556901.5587539819861744TruSeq Adapter, Index 13 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT684160.684974708918788No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCGCGTAT665230.6660221667651505TruSeq Adapter, Index 13 (97% over 40bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC312570.31294221346869966No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA297000.2973536724580216No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT269800.2701212822531118No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC253850.2541522887322181No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA169090.16929135513780091No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA160240.1604308164130417No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG149190.14936765789229708No Hit
CTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGC140150.14031689291243005No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT137540.13770378488173834No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA136380.13654240353476424No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG134340.13449997426939603No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC131130.13128615174889013No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC127750.12790212678960355No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA125210.12535910211605683No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC124720.12486851861604191No Hit
GCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCA119460.11960225492200424No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA111270.11140250213604061No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT107440.1075679413093934No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG106710.10683707201345281No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGGAT103590.10371335666641904TruSeq Adapter, Index 13 (97% over 40bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC101770.10189118938064934No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA101440.10156079641125154No Hit
GTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCC99940.10005901018671608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG29050.082.930541
AATCGGA33450.072.455762
GAATCCA142450.032.5196234
GCAGAAT145000.031.9783021
CACTAGC86550.031.7452854
TCACTAG89050.031.5869053
AGAATCC145150.031.415423
AGACTCA84700.030.6705932
TTCACTA94600.030.1456012
CAGAATC151250.029.793312
GTTCACT98300.029.7491171
CAACTGT60750.029.6229231
AATCCAG157250.029.5511575
ACTAGCA95050.029.2114945
ACTCAGA125100.028.2214134
CTAGCAA101700.027.087556
CAGACTC99700.026.7112031
TAGCAAC103900.026.5836357
GACTCAG100850.025.9501913
CTCAGAG190400.025.6871241
Produced by FastQC (version 0.11.5)