FastQCFastQC Report
Sat 10 Jun 2017
HNTYTBGX2_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTYTBGX2_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294770769
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29120140.9878910347450361No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG3043370.10324531195289584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGG1394250.020.8572676
TATACCG1514400.020.6899875
GTTATAC1482300.020.2206613
GGACCAT723400.019.5220346
TTATACC1582550.018.8198954
ACCGGTG1543150.018.4508138
CCGGTGG1536500.017.9453169
TACCGGT1699800.017.7401717
GGCTTCA1550700.017.64327214
TGGACCA894650.017.3822125
TTCAGGG1560300.017.33396117
TCAGGGG1607350.017.28220718
GGTATTC1351000.017.02249124
TGGCTTC1640250.016.87685813
GGGTATT1360650.016.8111623
CGGGTCC286050.016.7299375
TGTTATA1940200.016.5384242
GGGGTAT1385900.016.49681722
CGCCCTA178400.016.3517327
GGGTCCA266150.016.0315726
Produced by FastQC (version 0.11.2)