FastQCFastQC Report
Sat 10 Jun 2017
HNTYTBGX2_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTYTBGX2_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294770769
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG3319640.11261767953660289No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCT4139750.013.8840961
CCTACAA4026050.013.88025951
TTACCGT4243350.013.8758871
TTCCAGA7112600.013.8754521
CCTCTCT3168500.013.874834
ACACCTT4148000.013.8652572
CCACACT3734000.013.8525172
TACTCCA3508500.013.8507633
CACCTTC4146400.013.8501363
TCAACCG2960700.013.8396431
TCAGAAC3860100.013.8372732
TTCCACC2335050.013.8339362
CCTTCTT3260650.013.832971
TCGACCA3386450.013.831582
TTCGACC3370800.013.8301571
AATCCTT3910150.013.8291772
CACAGAA6894450.013.8267771
AACTACC3853450.013.8217526
CTACTCC3524750.013.8146352
CCTTCGT4145500.013.8130755
Produced by FastQC (version 0.11.2)